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thermal cycler abi stepone plustm real-time pcr system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher thermal cycler abi stepone plustm real-time pcr system
    Thermal Cycler Abi Stepone Plustm Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+cycler+abi+stepone+plustm/thermal+cycler+abi+stepone+plustm+real+time+pcr+system/us11479773-301-8-9
    Average 90 stars, based on 1 article reviews
    thermal cycler abi stepone plustm real-time pcr system - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Diagnosis and identification ofLeishmaniaspp. from Giemsa-stained slides, by real-time PCR and melting curve analysis in south-west of Iran
    Article Snippet: Real-time PCR amplification and detection were conducted with fluorescent SYBR Green I in a reaction volume of 20 ml, including (5 ml) extracted DNA, 15 ml of reaction mixture containing 4 mM of MgCl2, 0.5 mM primers and 2 ml of LightCycler FastStart DNA Master SybrGreen I buffer (Roche Applied Science, Meylan, France). .. DNA was amplified using thermal cycler (ABI StepOne PlusTM) under the following conditions: 4 minutes at 95uC, followed by 35 cycles of 10 seconds at 95uC, 10 seconds at 62uC and 10 seconds at 72uC with watching of fluorescence at the end of elongation step. ..

    Fluorescence:

    Article Title: Diagnosis and identification ofLeishmaniaspp. from Giemsa-stained slides, by real-time PCR and melting curve analysis in south-west of Iran
    Article Snippet: Real-time PCR amplification and detection were conducted with fluorescent SYBR Green I in a reaction volume of 20 ml, including (5 ml) extracted DNA, 15 ml of reaction mixture containing 4 mM of MgCl2, 0.5 mM primers and 2 ml of LightCycler FastStart DNA Master SybrGreen I buffer (Roche Applied Science, Meylan, France). .. DNA was amplified using thermal cycler (ABI StepOne PlusTM) under the following conditions: 4 minutes at 95uC, followed by 35 cycles of 10 seconds at 95uC, 10 seconds at 62uC and 10 seconds at 72uC with watching of fluorescence at the end of elongation step. ..



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